auto
Find a product
Back to results

Polyclonal Rabbit anti-CASP9 antibody Abcam #ab2325

Polyclonal Rabbit anti-CASP9 antibody

  • Company: Abcam
  • Product code: ab2325
  • Review rating:1.00 (1 review)
  • Verified publication: 1

Product Details

1DB_ID: 1DB-001-0000107168
Company: Abcam
Company product code: ab2325
Product name: Anti-active Caspase 9 antibody
Product type: Primary, Antibody
Size/Price: 50 µg/
Target: CASP9
Tested applications: ICC, WB
Host: Rabbit
Reacts with: Mouse, Rat
Clonality: Polyclonal
Description: Rabbit polyclonal to active Caspase 9
Immunogen: Synthetic peptide from the cleavage site of rat caspase-9
Specificity: This antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase.
Storage Buffer: Preservative: 0.02% Thimerosal (merthiolate) Constituents: 50% Glycerol, 1% BSA, PBS
Purity: Affinity purified
Isotype: IgG
Concentration: 50 µg at 0.2mg/ml
Storage instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Primary Antibody Notes: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Alternative Names: APAF 3 antibody;APAF3 antibody;Apoptosis related cysteine peptidase antibody
Relevance: Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation

Reviews (1)

1 - Unpublishable
Application: WB
Sample: Human Lysates
Buffer: PBS
Dilution: 1/1000
See all reviews

Verified publications (1)

Cancer Cell, 2009
NF-kappaB (RelA) is constitutively active in many cancers, where it upregulates antiapoptotic and other oncogenic genes. While proinflammatory ...
See all publications

1DegreeBio goes to great lengths to ensure the accuracy of the product information listed on the site, but we still encourage you to check with vendors to confirm product details before purchasing.