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Polyclonal Rabbit anti-CASP9 antibody Abcam #ab2325

Polyclonal Rabbit anti-CASP9 antibody

  • Company: Abcam
  • Product code: ab2325
  • Review rating:1.00 (1 review)
  • Verified publication: 1

Product Details

1DB_ID: 1DB-001-0000107168
Company: Abcam
Company product code: ab2325
Product name: Anti-active Caspase 9 antibody
Product type: Primary, Antibody
Size/Price: 50 µg/
Target: CASP9
Tested applications: ICC, WB
Host: Rabbit
Reacts with: Mouse, Rat
Clonality: Polyclonal
Description: Rabbit polyclonal to active Caspase 9
Immunogen: Synthetic peptide from the cleavage site of rat caspase-9
Specificity: This antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase.
Storage Buffer: Preservative: 0.02% Thimerosal (merthiolate) Constituents: 50% Glycerol, 1% BSA, PBS
Purity: Affinity purified
Isotype: IgG
Concentration: 50 µg at 0.2mg/ml
Storage instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Primary Antibody Notes: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Alternative Names: APAF 3 antibody;APAF3 antibody;Apoptosis related cysteine peptidase antibody
Relevance: Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation

Reviews (1)

1 - Unpublishable
Application: WB
Sample: Human Lysates
Buffer: PBS
Dilution: 1/1000
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Verified publications (1)

Cancer Cell, 2009
NF-kappaB (RelA) is constitutively active in many cancers, where it upregulates antiapoptotic and other oncogenic genes. While proinflammatory ...
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