|Company product code:||A303-609A|
|Product name:||Goat anti-Phospho ERK1/2 (T202/Y204, T185/Y187) (T202/Y204, T185/Y187) Antibody, Affinity Purified|
|Product type:||Primary, Antibody|
Price ranges, USD:
|Description:||p42-MAPK antibody, ERT1 antibody, MAPK3 antibody, PRKM1 antibody, PRKM2 antibody|
|Immunogen:||A synthetic peptde corresponding to a region of human Extracellular Signal-Regulated Kinase-1 surrounding threonine 202 and tyrosine 204 (when using the numbering according to NCBI entry NP_002737.2, GeneID 5595) and human Extracellular Signal-Regulated Kinase-2 surrounding threonine 185 and tyrosine 187 (when using the numbering according to NCBI entry NP_620407.1, Gene ID 5594) when the threonine and tyrosine residues are phosphorylated.|
|Storage Buffer:||2 - 8° C|
|Storage instructions:||Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide|
Detection of Human Phospho-ERK1 and ERK2 by Immunoprecipitation (IP). Samples: 1 mg whole cell lysate was IP’d from HeLa cells that were mock treated (-) or treated with TPA (+); 20% of the IP was loaded. Antibodies: In (A), (B), and (C), affinity purified goat anti-Phospho ERK1/2 A303-609A was used for IP (6ug/1mg lysate). In (A) Rabbit anti-ERK1 (A302-060A) was used for western blot (WB) at 1 µg/ml. In (B) Rabbit anti-ERK2 (A302-061A) was used for (WB) at 1 µg/ml. In (C) a mix of anti-ERK1 (A302-060A) and anti-ERK2 (A302-061A) was used for WB at 1 µg/ml each. Detection: Chemiluminescence with exposure times of 30 seconds for (A), (B), and (C).
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