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Polyclonal Goat anti-MAPK3 antibody Bethyl Laboratories #A303-609A

Polyclonal Goat anti-MAPK3 antibody

Product Details

1DB_ID: 1DB-001-0001242853
Company: Bethyl Laboratories
Company product code: A303-609A
Product name: Goat anti-Phospho ERK1/2 (T202/Y204, T185/Y187) (T202/Y204, T185/Y187) Antibody, Affinity Purified
Product type: Primary, Antibody
Size/Price: 0.1 ml/
Target: MAPK3
Tested applications: IP
Host: Goat
Reacts with: Human
Clonality: Polyclonal
Modification: Phosphorylated
Description: p42-MAPK antibody, ERT1 antibody, MAPK3 antibody, PRKM1 antibody, PRKM2 antibody
Immunogen: A synthetic peptde corresponding to a region of human Extracellular Signal-Regulated Kinase-1 surrounding threonine 202 and tyrosine 204 (when using the numbering according to NCBI entry NP_002737.2, GeneID 5595) and human Extracellular Signal-Regulated Kinase-2 surrounding threonine 185 and tyrosine 187 (when using the numbering according to NCBI entry NP_620407.1, Gene ID 5594) when the threonine and tyrosine residues are phosphorylated.
Storage Buffer: 2 - 8° C
Purity: Affinity purified
Isotype: IgG
Concentration: 1 mg/ml
Storage instructions: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Gene ID: 5595
Potein ID: NP_002737.2

Reviews (1)

3 - Good (Publishable / Would use again)
Application: WB
Sample: 293 cell lysate
Buffer: 1%BSA/PBS
Dilution: 1/2000
See all reviews

Quality control data

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Detection of Human Phospho-ERK1 and ERK2 by Immunoprecipitation (IP). Samples: 1 mg whole cell lysate was IP’d from HeLa cells that were mock treated (-) or treated with TPA (+); 20% of the IP was loaded. Antibodies: In (A), (B), and (C), affinity purified goat anti-Phospho ERK1/2 A303-609A was used for IP (6ug/1mg lysate). In (A) Rabbit anti-ERK1 (A302-060A) was used for western blot (WB) at 1 µg/ml. In (B) Rabbit anti-ERK2 (A302-061A) was used for (WB) at 1 µg/ml. In (C) a mix of anti-ERK1 (A302-060A) and anti-ERK2 (A302-061A) was used for WB at 1 µg/ml each. Detection: Chemiluminescence with exposure times of 30 seconds for (A), (B), and (C).

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About Bethyl Laboratories

Dedicated to improving lives while supporting scientific discovery, Bethyl Laboratories has provided top-quality antibody products and services since 1972. We manufacture to exacting standards and validate our antibodies in a wide range of applications to help researchers accelerate discovery. For special needs, we offer the market's most experienced custom antibody service.