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NotI-HF™ New England Biolabs (NEB) #R3189

NotI-HF™

Product Details

1DB_ID: 1DB-001-0001357739
Company: New England Biolabs (NEB)
Company product code: R3189
Product name: NotI-HF™
Product type: Enzyme
Multiple sizes available: 500 units / ; 2500 units /
Enzyme name: NotI-HF
Tested applications: Nucleic Acid Digestion
Source: E. Coli
Concentration: 20,000 units/ml and 100,000 units/ml
Storage instructions: -20°C
Storage Condition: 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C
Product Description: New England Biolabs provides customers with high quality tools for a wide range of molecular biology applications. As part of our ongoing commitment to the study and improvement of restriction enzymes, we are pleased to introduce a line of restriction enzymes that have been engineered for maximum performance, convenience and flexibility. These engineered enzymes have the same specificity as their established counterparts with the benefit of reduced star activity. In addition, all of these engineered enzymes work optimally in NEBuffer 4, which has the highest level of enzyme compatibility, and will simplify double digest reactions. They are all Time-Saver qualified, and digest substrate DNA in five minutes.
Source Detail: An E. coli strain that carries the cloned and modified (K150A) NotI gene from Nocardia otitidis-caviarum (ATCC 14630)
Usage Note: NotI-HF™ has the same specificity of NotI (NEB #R0189), but it has been engineered for reduced star activity.

Reviews (2)

4 - Excellent (Publishable / Ideal reagent)
Application: Cloning
4 - Excellent (Publishable / Ideal reagent)
Application: Cloning
See all reviews

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