|Company product code:||A302-060A|
|Product name:||Rabbit anti-ERK1 Antibody, Affinity Purified|
|Product type:||Primary, Antibody|
|Size/Price:||0.1 ml (20 - 100 blots)/
Price ranges, USD:
|Tested applications:||IF, IHC, IP, WB, PLA|
|Description:||p44-MAPK antibody, ERT2 antibody, p44-ERK1 antibody, MAPK 1 antibody, microtubule-associated protein kinase 2 antibody, PRKM3 antibody|
|Immunogen:||A synthetic peptde corresponding to a region between residue 329 and 379 of human extracellular signal-related kinase 1 (mitogen-activated protein kinase 3) using the numbering given in entry NP_002737.2 (GeneID 5595).|
|Storage Buffer:||2 - 8° C|
|Storage instructions:||Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide|
Detection of Human ERK1 by Western Blot and Immunoprecipitation. Samples:Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies:Affinity purified rabbit anti-ERK1 antibody A302-060A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection:Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of Human ERK1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK1 (Cat. No. A302-060A) used at a dilution of 1:200 (1 Detection: DAB
This antibody is qualified for the Proximity Ligation Assay (PLA).PLA uses paired antibodies, so please refer to the PLA page of our website for specific imaging obtained by pairing this antibody with other qualified antibodies. The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
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