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Polyclonal Rabbit anti-MAPK3 antibody Bethyl Laboratories #A302-060A

Polyclonal Rabbit anti-MAPK3 antibody

Product Details

1DB_ID: 1DB-001-0000142812
Company: Bethyl Laboratories
Company product code: A302-060A
Product name: Rabbit anti-ERK1 Antibody, Affinity Purified
Product type: Primary, Antibody
Size/Price: 0.1 ml (20 - 100 blots)/
Target: MAPK3
Tested applications: IF, IHC, IP, WB, PLA
Host: Rabbit
Reacts with: Human
Clonality: Polyclonal
Description: p44-MAPK antibody, ERT2 antibody, p44-ERK1 antibody, MAPK 1 antibody, microtubule-associated protein kinase 2 antibody, PRKM3 antibody
Immunogen: A synthetic peptde corresponding to a region between residue 329 and 379 of human extracellular signal-related kinase 1 (mitogen-activated protein kinase 3) using the numbering given in entry NP_002737.2 (GeneID 5595).
Storage Buffer: 2 - 8° C
Purity: Affinity purified
Isotype: IgG
Concentration: 0.2 mg/ml
Storage instructions: Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide
Gene ID: 5595
Potein ID: NP_002737.2

Reviews (1)

3 - Good (Publishable / Would use again)
Application: WB
Sample: 293 cell lysate
Buffer: 1%BSA/PBS
Dilution: 1:2000
See all reviews

Quality control data

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Detection of Human ERK1 by Western Blot and Immunoprecipitation. Samples:Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies:Affinity purified rabbit anti-ERK1 antibody A302-060A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection:Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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Detection of Human ERK1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK1 (Cat. No. A302-060A) used at a dilution of 1:200 (1 Detection: DAB

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This antibody is qualified for the Proximity Ligation Assay (PLA).PLA uses paired antibodies, so please refer to the PLA page of our website for specific imaging obtained by pairing this antibody with other qualified antibodies. The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

1DegreeBio goes to great lengths to ensure the accuracy of the product information listed on the site, but we still encourage you to check with vendors to confirm product details before purchasing.

About Bethyl Laboratories

Dedicated to improving lives while supporting scientific discovery, Bethyl Laboratories has provided top-quality antibody products and services since 1972. We manufacture to exacting standards and validate our antibodies in a wide range of applications to help researchers accelerate discovery. For special needs, we offer the market's most experienced custom antibody service.