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Mouse anti-CASP3 antibody Santa Cruz Biotechnology, Inc. #sc-7272

Mouse anti-CASP3 antibody

Product Details

1DB_ID: 1DB-001-0000183844
Company: Santa Cruz Biotechnology, Inc.
Company product code: sc-7272
Product name: caspase-3 Antibody (E-8)
Product type: Primary, Antibody
Size/Price: 200 µg/ml/
Target: CASP3
clone: E-8
Tested applications: ELISA, FACS, IF, IHC, IP, WB
Host: Mouse
Reacts with: Human
Description: caspase-3 Antibody (E-8) is a mouse monoclonal IgG2a provided at 200 µg/ml raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA agarose conjugate for IP studies, sc-7272 AC, 500 µg/0.25 ml agarose HRP conjugate, sc-7272 HRP, 200 µg/ml fluorescein (sc-7272 fiTC) and rhodamine (sc-7272 TRITC) conjugates for immunofluorescence, 200 µg/ml phycoerythrin conjugate for intracellular FCM, sc-7272 PE, 100 tests Alexa Fluor® 405 (sc-7272 AF405), Alexa Fluor® 488 (sc-7272 AF488) and Alexa Fluor® 647 (sc-7272 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
Isotype: IgG2A
Background: Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Reviews (1)

3 - Good (Publishable / Would use again)
Application: WB
Sample: Huh-7 cell lusate
Buffer: 20mM Tris-HCl, pH 7.5, 150mM NaCl, 2mM EDTA, 2mM EGTA, 0.5% (v/v) Triton X-100, 2mM dithiothreitol, protease inhibitor cocktail Complete (Roche), 10mM NaF, 1 mM okadaic acid, 1mM Na vanadate
Dilution: 1/1000
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Verified publications (2)

Oncotarget, 2012
Head and neck squamous cell carcinomas (HNSCCs) are the sixth most common malignant neoplasm and more than 50% of patients succumb to this disease. ...
Evid Based Complement Alternat Med, 2010
The present study was carried out to evaluate the effects of the water extract of Chinese medicine "Pingliu Keli" (PK) on human glioma cell viability ...
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About Santa Cruz Biotechnology, Inc.

Santa Cruz Biotechnology, Inc. is focused on the ongoing development of research antibodies. It is their goal to continue to offer the broadest range of research reagents in the field. They provide superior, innovative primary antibodies and support products.