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Mouse anti-TP53 antibody Santa Cruz Biotechnology, Inc. #sc-126

Mouse anti-TP53 antibody

Product Details

1DB_ID: 1DB-001-0000000528
Company: Santa Cruz Biotechnology, Inc.
Company product code: sc-126
Product name: p53 Antibody (DO-1)
Product type: Primary, Antibody
Size/Price: 200 µg/ml/
Target: TP53
clone: DO-1
Tested applications: FACS, IF, IHC, IP, WB
Host: Mouse
Reacts with: Human
Description: p53 Antibody (DO-1) is a mouse monoclonal IgG2a provided at 200 µg/ml N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin recommended for detection of wt and mutant p53 of human origin under denaturing and non-denaturing conditions by WB, IP, IF, IHC(P) and FCM agarose conjugate for IP studies, p53 Antibody (DO-1) AC: sc-126 AC, 500 µg/0.25 ml agarose phycoerythrin (sc-126 PE), PerCP (sc-126 PerCP) and PerCP-Cy5.5 (sc-126 PCPC5) conjugates for intracellular FCM, 100 tests HRP conjugate, p53 Antibody (DO-1) HRP: sc-126 HRP, 200 µg/ml TransCruz reagent for Gel Supershift and ChIP applications, p53 Antibody (DO-1) X: sc-126 X, 200 µg/0.1 ml fluorescein (p53 Antibody (DO-1) fiTC: sc-126 fiTC) conjugate for immunohistochemistry, 200 µg/ml rhodamine (p53 Antibody (DO-1) TRITC: sc-126 TRITC) conjugate for immunofluorescence, 200 µg/ml Alexa Fluor® 405 (sc-126 AF405), Alexa Fluor® 488 (sc-126 AF488) and Alexa Fluor® 647 (sc-126 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
Isotype: IgG2A
Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Reviews (12)

4 - Excellent (Publishable / Ideal reagent)
Application: WB
3 - Good (Publishable / Would use again)
Application: IF
Sample: human breast cell line
Buffer: PBS
Dilution: 1/200
3 - Good (Publishable / Would use again)
Application: ICC
Sample: Human cell line
Buffer: 1% BSA in PBS
Dilution: 1/100
3 - Good (Publishable / Would use again)
Application: WB
Sample: human cell lysate
Buffer: 5% BSA in TBS
Dilution: 1/500
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: Cytosol, nuclear and mitochondrial fractions of cancer cells
Buffer: 5% nonfat milk in TBST
Dilution: 1/1000
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: various lung cancer cell lines
Buffer: 5% nonfat milk in TBST
Dilution: 1/2000
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: A549, MCF7, H596 cells
Buffer: TBS
Dilution: 1/1000
3 - Good (Publishable / Would use again)
Application: WB
Sample: human ovarian cell lysate
Buffer: bsa
Dilution: 1/1000
3 - Good (Publishable / Would use again)
Application: WB
Sample: A549 cells
Buffer: RIPA
Dilution: 1/1000
2 - Poor (Publishable / Would seek higher quality in future)
Application: WB
Sample: Human cell lysate (293T cells)
Buffer: Cellytic
Dilution: 1:1000
2 - Poor (Publishable / Would seek higher quality in future)
Application: WB
Sample: Human Cell Lysate
Buffer: Cellytic
Dilution: 1:500
3 - Good (Publishable / Would use again)
Application: WB
See all reviews

Verified publications (3)

PLoS ONE, 2011
Tpr is a large coiled-coil protein located in the nuclear basket of the nuclear pore complex for which many different functions were proposed from ...
J. Virol., 2010
The E6 proteins from high-risk, cancer-causing types of human papillomavirus (HPV) are characterized by the presence of a PDZ (PSD95/Dlg/ZO-1) binding...
J. Cell. Sci., 2012
The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair ...
See all publications

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About Santa Cruz Biotechnology, Inc.

Santa Cruz Biotechnology, Inc. is focused on the ongoing development of research antibodies. It is their goal to continue to offer the broadest range of research reagents in the field. They provide superior, innovative primary antibodies and support products.