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Monoclonal Mouse anti-ACTB antibody United States Biological #A0760-40

Product Details

1DB_ID: 1DB-001-0000532857
Company: United States Biological
Company product code: A0760-40
Product name: Actin, beta
Product type: Primary, Antibody
Size/Price: 100ul/
Target: ACTB
clone: 2A2.1
Tested applications: Blocking, ELISA, IF, IHC
Host: Mouse
Reacts with: Human, Mouse, Rat, Rabbit, Chicken, Cat, Dog, Xenopus, Drosophila, Cow, Sheep, Guinea Pig
Clonality: Monoclonal
Recommended dilutions: Western Blot:1:5000. Useful as a loading control for a wide variety of cell extracts.
Immunocytochemistry: 1:1000
Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formailin-fixed tissue sections. acetone fixation. B5, Methacarn, ethanol or Bouin\'s solution may also be used as fixatives.
Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque.
Optimal dilutions to be determined by the researcher.
Specificity: Recognizes Xenopus beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech.
Storage Buffer: Supplied as a sterile-filtered, defibrinated neat serum, 0.1% sodium azide.
Storage instructions: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Additional info: Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. .

Category:
Abs to Actin

Grade:
Serum
Category: Abs to Actin
Grade: Serum

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: IF
Sample: mouse pancreas in OCT
Buffer: PBST + 10% FBS
Dilution: 1/100
See all reviews

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About United States Biological

United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience. United States Biological's biochemicals, antibodies, recombinant proteins, cell culture media and molecular biology kits are used in virtually all scientific applications and settings, including genomic research, biotechnology, pharmaceutical development and the diagnosis of disease. Products are available from affordable research quantities to larger bulk amounts for process development and production. Our customers include most of the leading pharmaceutical and biotechnology companies located in the US and abroad. 350,000+ antibodies biochemicals isotopes culture media recombinant and native proteins enzymes lectins research kits carbohydrates serum tissues cells cDNA cloning and more MetaboLyte? BioAssay? BioGenomics? MagDetect? BioSeparation? and? Free Molecular Biology Grade Salsa with every order.