|Company:||United States Biological|
|Company product code:||A0760-40|
|Product name:||Actin, beta|
|Product type:||Primary, Antibody|
Price ranges, USD:
|Tested applications:||Blocking, ELISA, IF, IHC|
|Reacts with:||Human, Mouse, Rat, Rabbit, Chicken, Cat, Dog, Xenopus, Drosophila, Cow, Sheep, Guinea Pig|
|Recommended dilutions:||Western Blot:1:5000. Useful as a loading control for a wide variety of cell extracts.
Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formailin-fixed tissue sections. acetone fixation. B5, Methacarn, ethanol or Bouin\'s solution may also be used as fixatives.
Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque.
Optimal dilutions to be determined by the researcher.
|Specificity:||Recognizes Xenopus beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech.|
|Storage Buffer:||Supplied as a sterile-filtered, defibrinated neat serum, 0.1% sodium azide.|
|Storage instructions:||May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.|
|Additional info:||Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton..
Abs to Actin
|Category:||Abs to Actin|
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