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Polyclonal Rabbit anti-GFP antibody Novus Biologicals #NB600-308

Polyclonal Rabbit anti-GFP antibody

Product Details

1DB_ID: 1DB-001-0000717889
Company: Novus Biologicals
Company product code: NB600-308
Product name: GFP Antibody
Product type: Primary, Antibody
Size/Price: 0.1mg/
Target: GFP
Tested applications: ChIP, ICC, IF, IHC, WB
Host: Rabbit
Reacts with: All Species
Clonality: Polyclonal
Immunogen: Green fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Recommended dilutions: Immunocytochemistry, Immunofluorescence 1:200-1:2000, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:500- 1:5000
Specificity: Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
Storage Buffer: 0.02 M K Phosphate, 0.15 Sodium Chloride, pH 7.2
Concentration: 1.03 mg/ml
Storage instructions: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Additional info:

Background:
This GFP antibody has been developed specifically for use in applications requiring a high titer and specificity with minimum background. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

Entrez Human ID:


Preservative:
0.01% Sodium Azide

Species Reactivity:
This antibody detects Green Fluorescent Protein.

Swisssprot Human ID:


Applications Note:
This GFP antibody is useful for Immunohistochemistry-Paraffin, Immunofluorescence, immunoprecipitation, Western Blot and Immunocytochemistry.

Note:


Gene Symbol:


Alternative Name:
anti-EGFP antibody, anti-Enhanced Green Fluorescence Protein antibody, anti-Enhanced Green Fluorescent Protein antibody, anti-Green Fluorescent Protein antibody, anti-Green Fluorescent Protein antibody, Anti-GFP antibody, anti-S65T-GFP antibody, anti-RS-GFP antibody, anti-YFP antibody
Background: This GFP antibody has been developed specifically for use in applications requiring a high titer and specificity with minimum background. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.
Preservative: 0.01% Sodium Azide
Species Reactivity: This antibody detects Green Fluorescent Protein.
Applications Note: This GFP antibody is useful for Immunohistochemistry-Paraffin, Immunofluorescence, immunoprecipitation, Western Blot and Immunocytochemistry.
Alternative Name: anti-EGFP antibody, anti-Enhanced Green Fluorescence Protein antibody, anti-Enhanced Green Fluorescent Protein antibody, anti-Green Fluorescent Protein antibody, anti-Green Fluorescent Protein antibody, Anti-GFP antibody, anti-S65T-GFP antibody, anti-RS-GFP antibody, anti-YFP antibody

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: IF
Sample: transgenic mouse pancreas in OCT
Buffer: 10% FBS in PBST
Dilution: 1/100
See all reviews

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About Novus Biologicals

Providing antibodies, proteins, peptides, lysates, kits and more to the bioscience community.