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Monoclonal Mouse anti-ACTB antibody Sigma Life Science #A5441

Monoclonal Mouse anti-ACTB antibody

  • Company: Sigma Life Science
  • Product code: A5441
  • Review rating:3.50 (3 reviews)
  • Verified publications: 8

Product Details

1DB_ID: 1DB-001-0000751457
Company: Sigma Life Science
Company product code: A5441
Product name: Monoclonal Anti-B-Actin antibody produced in mouse
Product type: Primary, Antibody
Target: ACTB
Tested applications: ELISA, IF, IHC, WB
Host: Mouse
Reacts with: Human, Mouse, Rat, Rabbit, Chicken, Cat, Dog, Pig, Sheep, Guinea Pig
Clonality: Monoclonal

Reviews (3)

3 - Good (Publishable / Would use again)
Application: ICC
Sample: Human cells
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/250
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: human cell lines
Buffer: TBST
Dilution: 1/5000
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: Huh cell lysate
Buffer: 20 mM Tris HCl pH= 7,4, 150 mM NaCl, 5 mM EDTA. 1% NP40 (now you can use IGEPAL CA630) , 2mM dithiothreitol, protease inhibitor cocktail Complete (Roche), 10 mM NaF, 1 mM okadaic acid, 1 mM Na vanadate.
Dilution: 1/10000
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Verified publications (8)

J. Comp. Neurol., 2011
Pcp4/pep19 is a modulator of Ca(2+) -CaM, a key molecule for calcium signaling, expressed in postmitotic neuroectoderm cells during mouse ...
J. Comp. Neurol., 2009
Numerous central nervous system (CNS) disorders share a common pathology in dysregulation of gamma-aminobutyric acid (GABA) inhibitory signaling. ...
J. Comp. Neurol., 2008
Rats lesioned with 6-hydroxydopamine (6-OHDA) as neonates exhibit behavioral and neurochemical abnormalities in adulthood that mimic Lesch-Nyhan ...
J. Comp. Neurol., 2010
Clathrin-coated vesicles are known to play diverse and pivotal roles in cells. The proper formation of clathrin-coated vesicles is dependent on, and ...
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About Sigma Life Science

Sigma-Aldrich is a leading Life Science and High Technology company. Their biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical and other high technology manufacturing.