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Reviews for Monoclonal Mouse anti-ACTB antibody Sigma Life Science #A5441

Monoclonal Mouse anti-ACTB antibody

  • Company: Sigma Life Science
  • Product code: A5441
  • Review rating:3.50 (3 reviews)
  • Verified publications: 8
3 - Good (Publishable / Would use again)
Application: ICC
Sample: Human cells
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/250
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: human cell lines
Buffer: TBST
Dilution: 1/5000
4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: Huh cell lysate
Buffer: 20 mM Tris HCl pH= 7,4, 150 mM NaCl, 5 mM EDTA. 1% NP40 (now you can use IGEPAL CA630) , 2mM dithiothreitol, protease inhibitor cocktail Complete (Roche), 10 mM NaF, 1 mM okadaic acid, 1 mM Na vanadate.
Dilution: 1/10000

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About Sigma Life Science

Sigma-Aldrich is a leading Life Science and High Technology company. Their biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical and other high technology manufacturing.