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Monoclonal Rat anti-CD4 antibody BioLegend #100427

Product Details

1DB_ID: 1DB-001-0000838229
Company: BioLegend
Company product code: 100427
Product name: Pacific Blue™ anti-mouse CD4
Product type: Primary, Antibody
Multiple sizes available: 25 µg / ; 100 µg /
Target: CD4
Tested applications: FACS
Host: Rat
Reacts with: Mouse
Clonality: Monoclonal
Conjugation: Pacific Blue™
Immunogen: Mouse CTL clone V4
Recommended dilutions: This CD4 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is 1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG2B
Fraction: Kappa
Concentration: 0.5 mg/ml
Storage instructions: The CD4 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells. Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-cell to sperm cell adhesion1,4, immunohistochemical staining9,10 of acetone-fixed frozen sections, and immunoprecipitation1,2. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody (Cat. No. 100506), but not RM4-4 antibody (Cat. No. 116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 100416).
Additional Protein Information: CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Reviews (3)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Murine Splenocytes
Buffer: HBSS w/ 2% FBS
Dilution: 1:100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse C57Bl6/J Splenocytes
Buffer: PBS
Dilution: 1:100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Lymphocytes
Buffer: PBS + 2% Serum
Dilution: 1/100
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Quality control data


Verified publications (11)

J. Exp. Med., 1991
A new, numerically minute population of cells representing the earliest T precursor cells in the adult mouse thymus has recently been isolated. This ...
J. Immunol., 1983
Monoclonal antibody GK1.5 recognizes a previously undescribed murine T cell surface molecule, designated L3T4, which migrates on SDS-PAGE under ...
Am. J. Respir. Cell Mol. Biol., 1994
The pathogenesis of asthma remains unclear. An in vivo murine model of antigen-induced airway hyperreactivity and inflammation was developed to ...
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Pacific Blue™ are registered trademarks of Molecular Probes Inc.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.