|Company product code:||100531|
|Product name:||Pacific Blue™ anti-mouse CD4|
|Product type:||Primary, Antibody|
|Multiple sizes available:||100 µg /
; 25 µg /
Price ranges, USD:
Price ranges, USD:
|Immunogen:||BALB/c mouse thymocytes|
|Recommended dilutions:||This CD4 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is 1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.|
|Storage Buffer:||Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.|
|Storage instructions:||The CD4 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Additional Application Information:||Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, immunohistochemistry of acetone-fixed frozen tissue sections2,3. The RM4-5 antibody blocks the binding of GK1.5 antibody (Cat. No. 100402) and H129.19 (Cat. No. 130302) to CD4 T cells, but not RM4-4 antibody (116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 100520).|
|Additional Protein Information:||CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.|
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Pacific Blue™ are registered trademarks of Molecular Probes Inc.