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Monoclonal Rat anti-CD4 antibody BioLegend #100531

Product Details

1DB_ID: 1DB-001-0000838258
Company: BioLegend
Company product code: 100531
Product name: Pacific Blue™ anti-mouse CD4
Product type: Primary, Antibody
Multiple sizes available: 100 µg / ; 25 µg /
Target: CD4
Tested applications: FACS
Host: Rat
Reacts with: Mouse
Clonality: Monoclonal
Conjugation: Pacific Blue™
Immunogen: BALB/c mouse thymocytes
Recommended dilutions: This CD4 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is 1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG2A
Fraction: Kappa
Concentration: 0.5 mg/ml
Storage instructions: The CD4 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, immunohistochemistry of acetone-fixed frozen tissue sections2,3. The RM4-5 antibody blocks the binding of GK1.5 antibody (Cat. No. 100402) and H129.19 (Cat. No. 130302) to CD4 T cells, but not RM4-4 antibody (116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 100520).
Additional Protein Information: CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Reviews (2)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Lymph node cells (mouse C57BL6/J)
Buffer: DMEM
Dilution: 1:100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Murine Lymph Node C57/Bl6
Buffer: HBSS w/ 2% FBS
Dilution: 1:50
See all reviews

Quality control data

Verified publications (4)

Blood, 2007
The T-cell protein tyrosine phosphatase (TC-PTP) is a negative regulator of the Jak/Stat cytokine signaling pathway. Our study shows that the absence ...
Blood, 2006
Although typically considered a neurotransmitter, there is substantial evidence that serotonin (5-HT) plays an important role in the pathogenesis of ...
Improved in situ immunodetection of leukocytes on paraffin embedded mouse spleen
Infect. Immun., 2003
Cells of the dendritic cell (DC) lineage, by their unique ability to stimulate naive T cells, may be of crucial importance in the development of ...
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Pacific Blue™ are registered trademarks of Molecular Probes Inc.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.