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Monoclonal Rat anti-CD44 antibody BioLegend #103017

Product Details

1DB_ID: 1DB-001-0000838527
Company: BioLegend
Company product code: 103017
Product name: Alexa Fluor® 647 anti-mouse/human CD44
Product type: Primary, Antibody
Multiple sizes available: 25 µg / ; 100 µg /
Target: CD44
Tested applications: FACS, IF, IHC
Host: Rat
Reacts with: Human, Mouse, Monkey, Cat, Dog, Pig, Chimpanzee, Cow, Horse, Baboon
Clonality: Monoclonal
Conjugation: Alexa Fluor® 647
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Recommended dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.* Alexa Fluor®647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor®is a registered trademark of Molecular Probes, Inc. Alexa Fluor®dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG2B
Fraction: Kappa
Concentration: 0.5 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 103014).
Additional Protein Information: CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human keratinocytes
Buffer: PBS, BD Bioscience Cytofix/Cytoperm buffers
Dilution: 1:200
See all reviews

Quality control data


Verified publications (8)

Cancer Res., 2005
Enhanced drug efflux mediated by ABCB1 P-glycoprotein and related ATP-binding cassette transporters is one of several mechanisms of multidrug ...
Immunogenetics, 1982
A murine leukocyte surface glycoprotein (Mr = 95 000) has been defined by means of xenogeneic monoclonal antibodies. In normal hematopoietic tissues, ...
J Transl Med, 2009
Foxp3 has been suggested to be a standard marker for murine Tregs whereas its role as marker for human Tregs is controversial. While some reports have...
J. Cell Biol., 1997
Upon antigen contact, epidermal Langerhans cells (LC) and dendritic cells (DC) leave peripheral organs and home to lymph nodes via the afferent ...
See all publications

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Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.