auto
Find a product
Back to results

Monoclonal Rat anti-ITGA2 antibody BioLegend #108909

Product Details

1DB_ID: 1DB-001-0000839165
Company: BioLegend
Company product code: 108909
Product name: APC anti-mouse CD49b (pan-NK cells)
Product type: Primary, Antibody
Multiple sizes available: 25 µg / ; 100 µg /
Target: ITGA2
Tested applications: FACS
Host: Rat
Reacts with: Mouse
Clonality: Monoclonal
Conjugation: APC
Immunogen: IL-2-propagated NK1.1+ cells from C57BL/6 mice
Recommended dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgM
Fraction: Kappa
Concentration: 0.2 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections. The binding of DX5 antibody to splenic NK cells can be blocked by HMα2 antibody.
Additional Protein Information: DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen. The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro.

Reviews (6)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM+FCS
Dilution: 1/75
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM+FCS
Dilution: 1/50
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM
Dilution: 1/100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Murine C57/Bl6
Buffer: HBSS w/ 2% FBS
Dilution: 1:100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Murine Spleen
Buffer: HBSS w/ 2% FBS
Dilution: 1:100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Lymphocytes
Buffer: PBS + 2% Serum
Dilution: 1/50
See all reviews

Quality control data


Verified publications (6)

J. Immunol., 2001
DX5 mAb is a useful reagent because it stains NK cells from all mouse strains examined. We have identified the molecule recognized by DX5 mAb by using...
J. Immunol., 2004
Aging is associated with a decline in immune function, particularly within the T cell compartment. Because CD8(+) T cells are critical mediators of ...
J. Immunol., 2006
Primary biliary cirrhosis (PBC) is an autoimmune disease of the liver, characterized by lymphocytic infiltrates in portal tracts, selective ...
J. Immunol., 2010
During their final maturation in the medulla, semimature single-positive (SP) thymocytes downregulate activation markers and subsequently exit into ...
See all publications

1DegreeBio goes to great lengths to ensure the accuracy of the product information listed on the site, but we still encourage you to check with vendors to confirm product details before purchasing.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.