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Monoclonal Rat anti-CD19 antibody BioLegend #115533

Product Details

1DB_ID: 1DB-001-0000839391
Company: BioLegend
Company product code: 115533
Product name: PerCP/Cy5.5 anti-mouse CD19
Product type: Primary, Antibody
Multiple sizes available: 25 µg / ; 100 µg /
Target: CD19
Tested applications: FACS
Host: Rat
Reacts with: Mouse
Clonality: Monoclonal
Conjugation: Cy5.5™, PerCP
Recommended dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG2A
Fraction: Kappa
Concentration: 0.2 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, and costimulation (synergistic with anti-IgM) of B cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 115514).
Additional Protein Information: CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Reviews (6)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM+FCS
Dilution: 1/75
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM+FCS
Dilution: 1/200
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Blood
Buffer: DMEM
Dilution: 1/100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: mouse lymph node
Buffer: pbs+2%serum
Dilution: 1/100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Murine C57/Bl6 lymph node
Buffer: HBSS with 2% FBS
Dilution: 1:50
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: Mouse Lymphicytes
Buffer: PBS + 2% FBS
Dilution: 1/100-1/50
See all reviews

Quality control data

Verified publications (10)

J. Immunol., 2004
To interfere with host immune responses, some microbial pathogens produce proteins with the properties of superantigens, which can interact via ...
Nat. Immunol., 2005
Dendritic cells (DCs) regulate various aspects of innate immunity, including natural killer (NK) cell function. Here we define the mechanisms involved...
Nat. Immunol., 2007
The mechanism of B cell-antigen encounter in lymphoid tissues is incompletely understood. It is also unclear how immune complexes are transported to ...
J. Immunol., 2007
Transmethylation affects several cellular events, including T cell activation, and blockade of this pathway may curtail inflammatory/autoimmune ...
See all publications

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About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.