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Monoclonal Mouse anti-CD24 antibody BioLegend #311109

Product Details

1DB_ID: 1DB-001-0000842459
Company: BioLegend
Company product code: 311109
Product name: Alexa Fluor® 647 anti-human CD24
Product type: Primary, Antibody
Multiple sizes available: 25 tests / ; 100 tests /
Target: CD24
Tested applications: FACS
Host: Mouse
Reacts with: Human, Chimpanzee
Clonality: Monoclonal
Conjugation: Alexa Fluor® 647
Recommended dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor®647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor®is a registered trademark of Molecular Probes, Inc. Alexa Fluor®dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Isotype: IgG2A
Fraction: Kappa
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3, immunohistochemical staining of acetone-fixed frozen tissue sections.
Additional Protein Information: CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Reviews (7)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/25
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/50
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/250
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/500
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/1000
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
See all reviews

Quality control data

1DegreeBio goes to great lengths to ensure the accuracy of the product information listed on the site, but we still encourage you to check with vendors to confirm product details before purchasing.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.