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Monoclonal Rat anti-CCR5 antibody BioLegend #313713

Product Details

1DB_ID: 1DB-001-0000842651
Company: BioLegend
Company product code: 313713
Product name: Alexa Fluor® 700 anti-human CD195 (CCR5)
Product type: Primary, Antibody
Multiple sizes available: 25 µg / ; 100 µg /
Target: CCR5
Tested applications: FACS
Host: Rat
Reacts with: Human
Clonality: Monoclonal
Conjugation: Alexa Fluor® 700
Immunogen: CHO cells transfected with human CCR5
Recommended dilutions: This reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is 2.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor®700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor®700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.** Alexa Fluor®700 is a registered trademark of Molecular Probes, Inc. Alexa Fluor®700 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG2A
Fraction: Kappa
Concentration: 0.5 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1
Additional Protein Information: CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α MIP-1β and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.

Reviews (7)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/25
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/50
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/100
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/250
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/500
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/1000
4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral blood cells
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
See all reviews

Quality control data


Verified publications (1)

Circ Cardiovasc Genet, 2012
Although monocytes in peripheral blood are no longer considered to be a homogeneous population, associations between distinct monocyte subsets and ...
See all publications

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Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.