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Monoclonal Mouse anti-CD3 antibody BioLegend #344803

Product Details

1DB_ID: 1DB-001-0000844222
Company: BioLegend
Company product code: 344803
Product name: FITC anti-human CD3
Product type: Primary, Antibody
Multiple sizes available: 25 tests / ; 100 tests /
Target: CD3
Tested applications: FACS
Host: Mouse
Reacts with: Human, Chimpanzee
Clonality: Monoclonal
Conjugation: FITC
Recommended dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Isotype: IgG1
Fraction: Kappa
Storage instructions: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional Application Information: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Additional Protein Information: CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε one CD3γ one CD3δ one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: FACS
Sample: human peripheral B lymphocytes (red) vs. myeloid cells (blue)
Buffer: HBSS+1% BSA
Dilution: 1/100
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Quality control data

Verified publications (6)

Exp. Anim., 2000
In order to effectively use cynomolgus monkeys as animal models for human diseases, more than 300 anti-human monoclonal antibodies (mAbs) were studied...
Blood, 1988
The expression of cytoplasmic CD3 (CyCD3) was analyzed in 45 leukemias, five thymus cell samples, five peripheral blood (PB) samples, and ten cell ...
Blood, 1999
Human immunodeficiency virus-associated Hodgkin's disease (HIV-HD) displays several peculiarities when compared with HD of the general population. ...
Arthritis Res. Ther., 2005
Analysis of biomarkers in synovial tissue is increasingly used in the evaluation of new targeted therapies for patients with rheumatoid arthritis ...
See all publications

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About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.