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Monoclonal Hamster anti-CCL2 antibody BioLegend #505905

Product Details

1DB_ID: 1DB-001-0000845090
Company: BioLegend
Company product code: 505905
Product name: LEAF™ Purified anti-mouse/rat/human MCP-1
Product type: Primary, Antibody
Multiple sizes available: 50 µg / ; 500 µg /
Target: CCL2
Tested applications: Blocking, ELISA, IF, IHC, WB, ELISPOT
Host: Hamster
Reacts with: Human, Mouse, Rat
Clonality: Monoclonal
Immunogen: CHO-expressed, recombinant mouse MCP-1
Recommended dilutions: Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, a concentration range of 5-10 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Isotype: IgG
Concentration: 1 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Additional Application Information: ELISA or ELISPOT Capture1: The Purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The Purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. The LEAF™ Purified antibody is suggested for ELISPOT capture.Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit and click on the support section.Neutralization1,4,6: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse/rat MCP-1 bioactivity in vivo and in vitro (Cat. No. 505906). Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry5 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Additional Protein Information: Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Quality control data

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About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.