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Monoclonal Hamster anti-CCL2 antibody BioLegend #506001

Product Details

1DB_ID: 1DB-001-0000845094
Company: BioLegend
Company product code: 506001
Product name: Biotin anti-mouse MCP-1
Product type: Primary, Antibody
Multiple sizes available: 50 µg / ; 500 µg /
Target: CCL2
Tested applications: ELISA, Detection antibody, ELISPOT
Host: Hamster
Reacts with: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Immunogen: CHO-expressed, recombinant mouse MCP-1
Recommended dilutions: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Isotype: IgG
Concentration: 0.5 mg/ml
Storage instructions: The antibody solution should be stored undiluted at 4°C. Do not freeze.
Additional Application Information: ELISA or ELISPOT Detection1,2: The biotinylated 4E2/MCP antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody for the detection of mouse MCP-1. Additional reported applications (for the relevant formats) include: Western blotting1. The 4E2/MCP is a non-neutralizing antibody1.
Additional Protein Information: Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 4E2/MCP antibody reacts with mouse MCP-1. This antibody does not react with human MCP-1 and related mouse beta chemokines, TCA3 and MIP-1beta. The 4E2/MCP antibody can not neutralize the bioactivity of natural or recombinant MCP-1.

Quality control data

Verified publications (1)

Cereb. Cortex, 2012
Intrauterine infection exacerbates neonatal hypoxic-ischemic (HI) brain injury and impairs the development of cerebral cortex. Here we used low-dose ...
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About BioLegend

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications.