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Monoclonal Mouse anti-GFP antibody EMD Millipore #MAB3580

Product Details

1DB_ID: 1DB-001-0000851670
Company: EMD Millipore
Company product code: MAB3580
Product name: Anti-Green Fluorescent Protein
Product type: Primary, Antibody
Size/Price: 100 µL/
Target: GFP
Tested applications: ELISA, ICC, IHC, WB
Host: Mouse
Reacts with: All Species
Clonality: Monoclonal
Immunogen: GFP-BSA
Specificity: Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.
Isotype: IgG1
Storage instructions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Background: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Alternative Names: GFP, eGFP
Subcategory: Epitope Tags & General Use

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: IF
Sample: tg mouse pancreas in OCT
Buffer: 10% FBS in PBST
Dilution: 1/500
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Quality control data

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Verified publications (34)

FEBS Lett., 2008
We conducted a phenotypic cDNA screening using a T cell line-based assay to identify human genes that render cells resistant to human immunodeficiency...
Hum. Mol. Genet., 2008
The ubiquitin-proteasome system (UPS) is the principal protein degradation system that tags and targets short-lived proteins, as well as damaged or ...
J. Natl. Cancer Inst., 2010
BACKGROUND Secretory GTPases like Rab27B control vesicle exocytosis and deliver critical proinvasive growth regulators into the tumor ...
Mol. Biol. Cell, 2011
Arl13b, a ciliary protein within the ADP-ribosylation factor family and Ras superfamily of GTPases, is required for ciliary structure but has poorly ...
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About EMD Millipore

EMD Millipore Bioscience delivers new technologies & workflow solutions that enable researchers to understand complex biological systems & identify new targets.