||ICC, IP, WB
||Human, Mouse, Rat, Monkey, Chicken, Dog, Drosophila, Sheep, Amphibian
||Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.
||Stable for 1 year at 2-8°C from date of receipt.
||Human, mouse and rat.
Predicted to react in drosophila, monkey, canine, sheep, chicken, frog and starfish. Wide species cross-reactivity is expected.
||Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.
||Epigenetics & Nuclear Function
1DegreeBio goes to great lengths to ensure the accuracy of the product information listed on the site, but we still encourage you to check with vendors to confirm product details before purchasing.