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Polyclonal Rabbit anti-GFP antibody EMD Millipore #AB3080

Product Details

1DB_ID: 1DB-001-0000851856
Company: EMD Millipore
Company product code: AB3080
Product name: Anti-Green Fluorescent Protein
Product type: Primary, Antibody
Size/Price: 50 µg/
Target: GFP
Tested applications: ELISA, ICC, IHC, WB
Host: Rabbit
Reacts with: Vertebrate
Clonality: Polyclonal
Immunogen: Highly purified native GFP from Aequorea victoria.
Specificity: AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
Storage instructions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Background: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Alternative Names: GFP, eGFP
Subcategory: Epitope Tags & General Use

Reviews (1)

4 - Excellent (Publishable / Ideal reagent)
Application: WB
Sample: HEK293T cells
Buffer: TBS
Dilution: 1/2000
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Quality control data


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Verified publications (36)

J. Neurosci., 2011
A large number of genetic diseases have been associated with truncated or misfolded membrane proteins trapped in the endoplasmic reticulum (ER). In ...
Mol. Biol. Cell, 2011
Arl13b, a ciliary protein within the ADP-ribosylation factor family and Ras superfamily of GTPases, is required for ciliary structure but has poorly ...
Neuroscience, 2011
During the development of epilepsy in adult animals, newly generated granule cells integrate abnormally into the hippocampus. These new cells migrate ...
Autophagy, 2011
Atg8 is a yeast protein involved in the autophagic process and in particular in the elongation of autophagosomes. In mammals, several orthologs have ...
See all publications

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About EMD Millipore

EMD Millipore Bioscience delivers new technologies & workflow solutions that enable researchers to understand complex biological systems & identify new targets.