hMAVS was transfected in HEK293T cells and an IP was performed using 1ug of Ab. The antibody was incubated at 4 degrees overnight (~1ug/ per IP). 30ul of Recombinant Protein A Agarose (20365 ) was added for 1hr and samples were run on WB. The same antibody was also used to blot MAVS (1/1000). 30ul of Protein A agarose works great. IP worked great and protein was pulled down. Endogenous protein was also pulled down (lane 2). This review is for Protein A supplied with agarose for IP experiments.
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