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Review on Thermo Fisher Scientific #20365 Recombinant Protein A Agarose

Recombinant Protein A Agarose

  • Rating: 4
This review has been submitted for an application that has not been tested
Dec 31, 2012 by
  • Application: Protein purification
  • Application rating: 4 - Excellent (Publishable / Ideal reagent)

Other experiment details:

hMAVS was transfected in HEK293T cells and an IP was performed using 1ug of Ab. The antibody was incubated at 4 degrees overnight (~1ug/ per IP). 30ul of Recombinant Protein A Agarose (20365 ) was added for 1hr and samples were run on WB. The same antibody was also used to blot MAVS (1/1000). 30ul of Protein A agarose works great. IP worked great and protein was pulled down. Endogenous protein was also pulled down (lane 2). This review is for Protein A supplied with agarose for IP experiments.

Additional documents:

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Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies. We create value for our customers through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges and increase laboratory productivity.