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Review on Thermo Fisher Scientific #20398 Pierce Protein G Agarose

Pierce Protein G Agarose

  • Rating: 4
Dec 31, 2012 by
  • Application: Protein purification
  • Application rating: 4 - Excellent (Publishable / Ideal reagent)

Other experiment details:

Experiment shows that NLRX1 interacts with UQCRC2. This was validated by performing a co-precipitation (IP: FLAG,) in HEK293T. Lanes 2 and 3 show this interaction using two different constructs of NLRX1 [FLAG and SPA (3xFLAG epitope)]. Lane 1 serves as a negative control showing that the interaction is specific due to NLRX1 being present. Lysates were incubated overnight at 4°C with Protein G Agarose (20398) and 1ug of anti-FLAG (F1804). Beads were washed three times, each for 45 minutes with RIPA and resuspended in 6x SDSPAGE loading buffer. Lysates and immunoprecipitates were analyzed using standard western blot techniques. Ig= Antibody heavy chains. Protein A agarose works well at 30ul per IP. Agarose was washed with buffer prior to incubation with the lysate + AB. This review is for using 30ul Protein A agarose per IP for experiments.

Additional documents:

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